Sequence:Human ubiquitin K29 only mutant. Except K29 all other lysines are mutated to arginine.
MW: 8.5 kD
Concentration: 2mg/mL
Buffer: 50mM PBS, pH7.0, 150mM NaCl
Purity: > 95% by SDS-PAGE
Storage: Store at -20℃. Avoid multiple FREEZE/THAW cycles.
- 歡迎來到優碧生物!
- QQ:5161153
- 13844081467
- English
Ubiquitin (K29 only Mutant)(human) (rec.)
產品編號 | 規格 | 價格 | 貨期 | |
UBM-K29 | 1mg | ¥750 | 現貨 |
- 產品描述
- 技術特性
- 訂貨信息
- 資料下載
Ubiquitin (K29 only Mutant)(human) (rec.)
Cat. # UBM-K29
Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast ubiquitin, and 100% aa sequence identity shared between human and mouse ubiquitin. In mammals, four ubiquitin genes encode for two ubiquitin-ribosomal fusion proteins and two poly-ubiquitin proteins. Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a ubiquitin-activating (E1) enzyme, a ubiquitin-conjugating (E2) enzyme, and a ubiquitin ligase (E3). ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting.