Ubiquitinconjugating Enzyme H5a (UbcH5a), also known as Ubiquitinconjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT). Human UbcH5a/UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively. Human UbcH5a/UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs. UbcH5a/UBE2D1 has a conserved E2 catalytic core domain that contains an active site cysteine residue, and it interacts with a variety of HECT and RING finger Ubiquitin ligases (E3) to mediate the ubiquitination of specific target proteins. UbcH5a/UBE2D1 interacts with the E3, E6AP, to conjugate Ubiquitin to the tumor suppressor, p53. Additional protein targets of UbcH5a/UBE2D1 include cFos, RIP1, and HIF1. Pathologically, UbcH5a/UBE2D1 is implicated in protein degradation during cancer and immune responses.
Stock:
X mg/ml (X µM) in PBS pH 7.3, 1 mM DTT
MW:
17 kDa (17 kDa UBE2D1)
Purity:
> 95% by SDS-PAGE under non-reducing conditions and visualized by Colloidal Coomassie Blue stain
USE:Recombinant Human UbcH5a/UBE2D1 is a member of the Ubiquitinconjugating Enzyme (E2) family that receives Ubiquitin from a UbiquitinActivating Enzyme (E1) and subsequently interacts with a Ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial UbcH5a/UBE2D1 concentration of 0.11 μM.
Storage: Store at -80°C. Avoid multiple freeze-thaw cycles.